fastidiosa strain diversity, evolutionary relationships, and epidemiology is limited. Development of Simple Sequence Repeat (SSR) markers for discrimination of X. These results support NP303 (Plant Diseases), Component 2 (Biology, Ecology, Epidemiology, and Spread of Plant Pathogens and their Relationships with Hosts and Vectors), Problem Statement 2B (Plant-Microbe-Vector Interactions). These results facilitate basic studies on plant pathogen-vector biology and indicate potential steps in pathogen inoculation that may be target for interruption. fastidiosa upon sharpshooter inoculation. fastidiosa cells were observed in sharpshooter saliva introduced into plants upon feeding, allowing determination of initial plant cells encountered by X. fastidiosa was used by ARS researchers in the Crop Disease, Pests, and Genetics Research Unit in Parlier, CA to visualize bacterial cells introduced into plants following sharpshooter feeding. Confocal microscopy of green fluorescent protein labeled (GFP) X. fastidiosa is inoculated to plants by sharpshooters is poorly understood. fastidiosa in planta at the site of sharpshooter inoculation. Significant Activities that Support Special Target Populations This project was terminated Maand replaced by new project 5302-22000-008-00D. Determine the biochemical, physiological, genetic and mechanistic bases of resistance to Xf infection and subsequent disease development in Vitis and selected Prunus species. Characterize the complex host-pathogen-insect vector-environment interactions of these pathosystems. Approach (from AD-416) Determine the epidemiology of xylellae diseases in California. Progress 10/01/05 to 03/14/07 Outputs Progress Report Objectives (from AD-416) The overall objective of this project is to reduce losses due to xylellae diseases during crop production and to develop effective, sustainable Xylella fastidiosa disease and insect vector management strategies.
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