![]() ![]() This conversion leads after 1–2 h to labeling of neuron somata, because of diffusion of the converted Kaede from the synaptic terminals (Fig. In this line, Kaede protein is present in all PLL neurons, including in their synaptic endings, and can be converted in any chosen neuromast ( Fig. We determined the positions of the sensory neurons that innervate neuromasts of the primI, and of the primII/D, system by photoconverting neuromasts of either system in 4 dpf Huc:kaede transgenic larvae (Sato et al., 2006). ![]() derived neuromasts, and, in particular, it has not been described whether they are interspersed with the neurons of the primI system or whether they are segregated as an independent ganglion. is known about the sensory neu- right under the basal rons that innervate primII- and primD- Scale bars: 50 m. This result confirms that the D/II system originates from subperidermal cells present just posterior to the otic vesicle at 20.5. We observed labeling in all secondary primordia between 48 and 54 hpf, much like the case of the MO- ngn1 embryos (primII, six of eight primD, four of eight D1, eight of eight data not shown). Having established that primII/primD progenitor cells are present just under the postotic periderm in MO-ngn1 embryos at 20.5 hpf, we performed similar photoconversions in untreated cldn:gfp embryos. 4 C A – C correspond to the same embryo). 4 C ), also in primD in one case, and in D1 in the other three cases (Fig. We later observed converted cells in primII in all cases (Fig. Of four successful conversions, we observed red cells in D0 in all cases at 35 hpf (Fig. In a very small proportion of the embryos, GFP-positive primI cells can be detected despite the general Kaede fluorescence, and the region between primI and the otic vesicle can be targeted (Fig. performed the photoconversion at 20.5 hpf, when primI prepares to migrate. ![]() The otic vesicle is outlined with dashed lines, and the PLL ganglion is outlined with white dots. In all panels, anterior is to the left, and dorsal up. Inset, Converted cells in primD (dorsal is to the right). #PRIMORDIA ENDINGS PLUS#H, J, Merged green ( cldnb - gfp plus unconverted Kaede expression) and red fluorescence. G, I, Merged Nomarski and red fluorescence images. G–J, Photoconversion of subperidermal cells anterior and ventral to the PLL ganglion in 24 hpf cldnb-GFP embryos injected with Kaede mRNA at the one-cell stage, avoiding the ganglion (PLLg), results in the presence of converted neurons in the ganglion at 48 hpf ( H, J ). F, The neurons innervating L1 ( n ϭ 6 red circles) and LII.1 ( n ϭ 5 green) are intermingled in the same ganglion (dotted outline), although there is partial segregation along the dorsoventral direction, with neurons innervating L1 (red) located preferentially in the dorsal half of the ganglion. E, Two hours after conversion, four neurons are labeled in the ganglion (dotted outline). D, Synaptic endings in neuromast LII.1 of a 4 dpf Huc:kaede larva, a few minutes after photoconversion. In C, primII is migrating along L1 (arrow indicates direction of migration). A–C, cldnb:gfp embryo coinjected with Kaede mRNA and ngn1 -M0 was photoconverted at 20.5 hpf ( A ), when primI begins to migrate, and examined at 35 hpf ( B ) and 54 hpf ( C ). It won’t be a hair’s breadth less.Origin of secondary primordia and neurons. I’ll slice off his balls right up to his ass. Lacking enlightenment and appreciation for the seminal blessing, Panurge failed to move beyond the classical cycle of castration and cuckolding and castration, repeating endlessly:Īnd you know what I’ll do to him? Crow-god! What Saturn did to his father Coelus (Seneca foretold it of me, and Lactantius confirmed it), what Rhea did to Attis. From the eight-century BGC ancient Greek text of Hesiod’s Genealogy of the Gods ![]()
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